Which Reagents Will Achieve the Following Transformation
Constitutive promoters are used routinely to drive ectopic gene expression. In the present study a strain named Ochrobactrum EEELCW01 isolated previously was inoculated into paddy soils to investigate the effect of FeOB inoculation on the As migration and transformation in paddy soils.
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Superior knockdown with Lipofectamine RNAiMAX Transfection Reagent compared to competing siRNA transfection reagents.
. Iron-oxidizing bacteria FeOB could oxidize FeII and mediate biomineralization which provides the possibility for its potential application in arsenic As remediation. Here we carried out a systematic comparison of eight commonly used constitutive promoters SV40 CMV UBC EF1A PGK and CAGG for mammalian systems and COPIA and ACT5C for Drosophila systems. At both 10 nM and 1 nM p53 siRNA Lipofectamine RNAiMAX Transfection Reagent provides more effective knockdown relative to nontransfected cells than other RNAi reagents including siLentFect Bio-Rad DharmaFECT Dharmacon.
We also included in the comparison the TRE promoter which can be activated by the. A The merger of a site-selective photoredox bioconjugation method with bioorthogonal chemistry would rapidly generate structurally defined biohybrids directly from endogenous proteinsCat catalyst.
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